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Ozone therapy has been utilized and extensively studied for many decades altogether. Its effects are proven, consistent and with minimal side effects. Medical o3, used to disinfect and treat disease, has been around for over 150 years. Used to treat infections, wounds and multiple diseases, o3's effectiveness has been well-documented. It has been used to disinfect drinking water before the turn of the last century. Ozone was known to treat as many as 114 diseases.[11] ozone therapy has been in use since the 1800s and in 1896 the genius nikola tesla patented the first o3 generator in the us, later forming the “tesla ozone company.

”[12] during the first world war (1914-18) doctors familiar with o3's antibacterial properties, and with few other medical resources available to them applied it topically to infected wounds and discovered o3 not only remedied infection, but also had hemodynamic and anti-inflammatory properties. In the late 1980s, reports had emerged that german physicians were successfully treating hiv patients with 03-aht (autohemotherapy). There was then no pharmaceutical treatment for hiv and a pandemic was feared, so canadian authorities authorized the study to test safety and efficacy of 03-aht in aids patients. Ozone had shown promise in in vitro testing. Ozone was seen effective at disinfecting extracorporeal blood samples of hiv; unfortunately for aids patients, 03-aht proved to be an in vivo ineffective treatment.


Inactivation of bacteria, viruses, fungi, yeast and protozoa: ozone therapy disrupts the integrity of the bacterial cell envelope through oxidation of the phospholipids and lipoproteins. In fungi, o3 inhibits cell growth at certain stages. With viruses, the o3 damages the viral capsid and upsets the reproductive cycle by disrupting the virus-to-cell contact with peroxidation. The weak enzyme coatings on cells which make them vulnerable to invasion by viruses make them susceptible to oxidation and elimination from the body, which then replaces them with healthy cells.


Stimulation of oxygen metabolism: ozone therapy causes an increase in the red blood cell glycolysis rate. This leads to the stimulation of 2,3-diphosphoglycerate which leads to an increase in the amount of oxygen released to the tissues. Ozone activates the krebs cycle by enhancing oxidative carboxylation of pyruvate, stimulating production of atp. It also causes a significant reduction in nadh and helps to oxidize cytochrome c. There is a stimulation of production of enzymes which act as free radical scavengers and cell-wall protectors: glutathione peroxidase, catalase and superoxide dismutase. Production of prostacyline, a vasodilator, is also induced by o3

Activation of the immune system: ozone administered at a concentration of between 30 and 55 μg/cc causes the greatest increase in the production of interferon and the greatest output of tumor necrosis factor and interleukin-2. The production of interleukin-2 launches an entire cascade of subsequent immunological reactions.


Diabetic complications are attributed to the oxidative stress in the body, o3 was found to activate the antioxidant system affecting the level of glycemia. Ozone prevented oxidative stress by normalizing the organic peroxide levels by activating superoxide dismutase.] Ozone was found to completely inactivate the hiv in vitro, this action of o3 was dose-dependent. Concentration used for inactivation was found to be non-cytotoxic. The inactivation was owing to the reduction of the hiv p24 core protein. Ozone was also found to increase the host immunity by increasing the production of cytokine. In an in vitro study, it was observed that o3 is very effective in reducing the concentrations of acinetobacter baumannii, clostridium difficile and methicillin-resistant staphylococcus aureus in dry as well as wet samples, hence it can be used as a disinfectant.

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